Instrumentation available for supercritical fluid extraction (SFE) and supercritical fluid chromatography (SFC) includes pumping systems from ISCO (100D and 260D).

SFE reduces the time needed for the extraction and concentration of organic compounds. Supercritical fluids have solvent strengths approaching those of liquid solvents. The solvent strength of a supercritical fluid, which is directly related to its density, can be modified by changing the extraction pressure and temperature. Supercritical fluids have lower viscosities and higher solute diffusivities than liquid solvents, thus enhancing mass transfer. The use of supercritical fluids that are gases at room temperature (e.g., CO₂ and N₂O) makes sample concentration relatively simple.

Capillary SFC is a rapidly developing method for the separation and identification of organic compounds that lack sufficient volatility of thermal stability to be separated by gas chromatography.